A stack of2D images can be organized and manipulated as a 3D array. A stack typically contains a set of related 2D images, such as a movie loop or serial sections from a volume. Images in a stack can be animated at rates up to 100 frames per second. NIH Image can reconstruct a new 2D image perpendicular to the plane of the slices in a stack, and it can perform volume rendering for visualizing the internal structures of 3D images. Macros are available for performing various operations on all the slices in a stack.

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