Pretreatment of the Color Image Data for Fast Fourier Transform

Histologic sections usually are stained to highlight specific tissues using different types of staining techniques. For example, in the modified Goldner's trichrome staining, the calcified tissue, uncalcified new bone, and osteoid are stained green, red, and orange, respectively (Fig. 5). In order to quantitate tissue orientation using FFT, staining is required to provide the data set for 2D FFT. Several techniques are available for color extraction. Figure 5 shows an example of the color extraction using a color spectrum distance transform in RGB space.

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