Overview

IPLab is a toolkit for image acquisition, analysis, and visualization of image data (Fig. 8). Applications for IPLab include bio-medical imaging, fluorescence microscopy (FISH, GFP and Ca ++ /pH ratio imaging) confocal and 3D imaging, high-quality deconvolution with EPR (Exhaustive Photon Reassignment) and other deconvolution algorithms, and electron microscopy. It contains a variety of processing functions, including particle analysis. IPLab can also be used for image acquisition and device control, and it is often purchased with optical image acquisition hardware. For medical image processing, an optional package called

Medical Access Pack (Macintosh only) can be purchased. It includes many standard file formats in the medical imaging community and the capability to access files over Ethernet and Internet links.

This package can interface with and control many optical imaging instruments. It is geared to provide a complete laboratory imaging system with analysis software, precluding the need to convert or transfer the acquired data to another analysis system or package. It can capture, process, and display 8-, 16-, and 32-bit integer and floating-point data, and 16-, 24-, and 48-bit color data. The image data can be displayed in time-lapse and sequence animation, and viewed from a 3D perspective.

IPLab has a set of enhancement and analysis functions, such as filtering, contrast enhancement, pseudocoloring, intensity statistics, transforms, profile plots, and geometric registration. Its particle analysis functions can identify objects by intensity, size, and shape, and then count, label, and measure the objects.

A developer can create functions in two ways: using IPLab's scripting mechanism to create macros, or developing a subroutine in C or Pascal to create an extension software module.

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