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FIGURE 15 NIH Image display.

and maximum gray values of selected regions. It can also perform automated particle analysis and provides tools for measuring path lengths and angles. It supports spatial and image density calibration with user specified units. A tool palette supports editing of color and gray-scale images, including the ability to draw lines, rectangles, and text. Images or selected regions can be flipped, rotated, inverted, and rescaled. It supports multiple windows and eight levels of magnification.

NIH Image directly supports Data Translation and Scion frame grabber cards for capturing images or movie sequences using a TV camera. Other frame grabbers are supported via plug-in modules. It also supports QuickTime compatible video digitizers such as those built into "AV" Macs and the Power Mac 7500/8500. Acquired images can be shading-corrected and frame-averaged.

NIH Image can be customized using a built-in Pascal-like macro language, via externally compiled plug-in modules, or on the Pascal source code level. Example macros, plug-ins, and complete source code are available from the NIH Web site (http://rsb.info.nih.gov/nih-image/) or by anonymous FTP from zippy.nimh.nih.gov.

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