O

j— Si-oligonucleotide + | PNA TAG|—R -- hybridization [29]

I Ni + protein HisX6 tags-» coordination attachment [15], [59]

antibody/antigen pair/ PCR-product adsorption on poly-l-lysine coated microscope slides [44] [13]

a) Polyacrylamide gel b) agarose gel + aldehyde functionalized protein/ C'<;/'iVN-' oligonucleotide

hydrazine, |ae|/si-x'XVN'N^'Pr , ^ hydrate---x ^ oligonucleotide

C pyrrole H lr

N-oligonucleotide electrochemical* | silicon-Au \ J^

oxidation H V- _// j oligonucleotide silicon-Pt-agarose-streptavidin + 5'-biotin-DNA-»- streptavidin-biotin binding

(strept)avidin + biotinylated protein-(strept)avidin-biotin binding

electrospray deposition of charged products on an electrostatic substrate

saline

!— Si-nitrocellulose + dextran/inulin NaCI)^ giycoconjugate a) [45] a) [55]

X = several hetero-bifunctional cross-linkers: [61]

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