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Fig. 5.3.5. Dependence of the rate of reaction on the concentration of dienophile at fixed diene concentrations • 55, o 99, B 190, v 415 mM. Initial rates (Vim) were obtained by monitoring the UV absorbance over the first 5% of conversion.

Fig. 5.3.6. HPLC analysis of Diels-Alder reactions catalyzed by 49nt d-RNA and the enantiomeric 49nt l-ribozyme. Samples were at 210 nm. The light gray curve in the chro-matograms corresponds to the uncatalyzed background reaction that occurs in the absence

Fig. 5.3.6. HPLC analysis of Diels-Alder reactions catalyzed by 49nt d-RNA and the enantiomeric 49nt l-ribozyme. Samples were at 210 nm. The light gray curve in the chro-matograms corresponds to the uncatalyzed background reaction that occurs in the absence analyzed on a chiral column with UV detection of ribozyme.

biomolecular catalysts for the Diels-Alder reaction. It compares favorably with catalytic antibodies raised against transition state analogs and with ribozymes that contain modified bases [11].

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