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These results imply that the functional l-RNA and d-RNA sequences are folding into mirror-image shapes; this is consistent with the observed circular dichroism (CD) spectra of the functional 58-mer RNA sequences. CD spectra were recorded in 0.1 M NaCl and 10 mM sodium phosphate, pH 7.0, at 4 °C on a Jasco J-600 spectropolarimeter.

Fig. 3.4.5. Biological stability of an aptamer (d-oligonucleotide) and a spiegelmer (l-oligonucleotide) in human serum. Both oligonucleotides were incubated in buffered human serum and after the times indicated samples were taken and analyzed on a polyacrylamide gel [18]. After 24 s no full length aptamer could be detected whereas the spiegelmer was fully stable even after 60 h. (Reprinted with permission from the Nature Publishing Group, Nat. Biotechnol. 1996, 14, 1112-1115).

Fig. 3.4.5. Biological stability of an aptamer (d-oligonucleotide) and a spiegelmer (l-oligonucleotide) in human serum. Both oligonucleotides were incubated in buffered human serum and after the times indicated samples were taken and analyzed on a polyacrylamide gel [18]. After 24 s no full length aptamer could be detected whereas the spiegelmer was fully stable even after 60 h. (Reprinted with permission from the Nature Publishing Group, Nat. Biotechnol. 1996, 14, 1112-1115).

tion was observed for 31 of the 68 doped positions. On the basis of this information a 55-mer truncated vasopressin binder (see Appendix) was designed and synthesized in both enantiomeric configurations. The dissociation constants for both complexes D-oligonucleotide/D-vasopressin and L-oligonucleotide/ L-vasopressin were determined by equilibrium dialysis to be approximately 1 ^m. In a cell based assay antagonism was demonstrated for the L-oligonucleotide (spiegelmer) whereas a control sequence with the same base composition and similar secondary structure was almost inactive.

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