Kshv

Gamma Herpesviruses

Plate 4 Sub-families of Human Herpesviruses. (see page 8).

Plate 5 CryoEM imaging and 3D reconstruction HSV-6 capsid. (a) A gallery of cryoEM particle images of HHV-6 capsids. (b) Shaded surface representation of HHV-6 capsid reconstruction at 30 A resolution. The structure is color coded according to capsid radius so that the capsid shell is in yellow, the triplexes are in green, and the upper domains of the pentons and hexons are in purple. (see page 17).

Plate 6 Comparison of HSV-1, HCMV, and KSHV capsids. A penton and a hexon are extracted from the cryoEM structures for detail comparison. Overall structure is similar; minor differences in hexon morphology may be a result of different structures of the distally located SCPs. (Modified from a figure by P. Lo). (see page 18).

Plate 7 Indirect immunofluorescence assay of HHV-6-infected cells. The HSB-2 cells infected with HHV-6A (stem GS) were stained with monoclonal antibody for IE1 (a) U27 (b) or gB (c) at 86 h p.i. (a) IE1 locates in nucleus with punctuated pattern. (b) U27 locates in nucleus hke forming replication compartment. (c) gB locates ;n the cytoplasm. (see page 49).

Plate 8 Cellular reactions in HHV-6-infected cells. Top Row: HHV-6A-infected HSB2 cells in culture: blastic transformation of infected cells (left) and nuclear inclusions in semithin section (right). Center Row: Prominent giant cell formation after infection with HHV-6A: SupT1 cells in culture (left) and L-428 Hodgkin cell line (semithin section of culture; right). Bottom Row: Antigen expression in HHV-6A-infected SupT1 cells: p41 early antigen (left) and HAR3 (i.e. mixture of late gp antigens; right). All APAAP immunohistochemistry. (see page 136).

Plate 9 Some characteristic tissue reactions in HHV-6 infections. Top Row: Blastic transformation of cells in tonsils with prominent nucleoli (left) with expression of HHV-6 p41 early antigen (red cells in APAAP reaction; right). Upper Center Row: Prominent apoptosis of HHV-6 infected cells in Kikuchi's lymphadenitis (left); hematopoietic stem cells in bone marrow expressing HHV-6 p41 antigen (red dots in APAAP reaction; right). Lower Center Row: Expression of HHV-6 gp135 in epithelial cells of the salivary gland (lip biopsy; left); HHV-6A-associated lymphoid interstitial pneumonitis, LIP, in an HIV infected patient (red cells carry p41 HHV-6 antigen; APAAP reaction). Bottom Row: Acute necrotizing encephalitis with HHV-6 p41 and DNA expression in numerous astroglial cells (red cells by APAAP left, black dots for HHV-6 DNA by in situ hybridization (right; Wagner et al., 1997). (see page 140).

Plate 10 Facial edema and an exfoliative dermatitis in DRESS. (see page 152).

Plate 11 Typical skin eruption observed in patient with primary HHV-6 infection. Maculopapular skin rash is observed on the trunk and extremity. (see page 164).

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