Index

A-actinin, 215, 221 AA see ascorbic acid 7-AAD, 314-15, 318 Acid Tyrodes, 43 acidic fibroblast growth factor (aFGF), 188, 189 aFGF see acidic fibroblast growth factor AFT024 stromal cells, 289, 290, 294, 296 cardiomyocytes, 224 embryoid bodies, 151 in vivo characterization, 124-5, 128-30, 133-6, 138-9, 145-6 alanyl-glutamine, 25-6 alizarin red, 253, 258, 263, 267 alkaline phosphatase, 253, 257-9, 263, 267, 268-9 pancreatic cells, 199-200, 205 spinal motor neurons, 349, 353-4, 355...

List of Contributors

Please note that many contributors have multiple affiliations these are listed in full at the start of their respective chapters. National Research Institute for Child Health and Development Centre for Stem Cell Biology University of Sheffield Western Bank Sheffield S10 2TN UK Department of Genetics The Hebrew University Jerusalem 91904 Israel McMaster Stem Cell and Cancer Research Institute Faculty of Health Sciences McMaster University Hamilton, Ontario L8N 3Z5 Canada Whitehead Institute for...

Antibodies

Useful antibodies are summarized in Table 1 but a number of them warrant further comment. The author recommends Jackson Immunoreseach Laboratories for all secondary antibodies. 5 days Activin A (10-100 ng ml) Low serum or serum free 5-10 days Retinoic Acid (0.1-5 M) FGF (10- 100 ng ml) Shh inhibitor ( ) , 7-21 days No FGF Notch inhibitor ( ) 7-21 days Nicotinamide (1-10 mM) Betacelluin (1-100 ng ml) HGF (10-100 ng ml) Exendin4 (1-100 ng ml) Figure 1 A schematic diagram of the f-cell...