Demonstrationblood Slide Preparation

To prepare a stained blood slide, follow these steps:

1. Clean two microscope slides with a cleaning agent (such as borax) and water, and thereafter avoid touching their flat surfaces.

2. Thoroughly wash hands with soap and water and dry them with paper towels.

3. Cleanse the end of the middle finger with some sterile cotton moistened with 70% alcohol and let the finger dry in the air.

4. Remove a sterile disposable blood lancet from its package without touching the sharp end.

5. Puncture the skin on the side near the tip of the middle finger with the lancet and properly discard the lancet.

6. Wipe away the first drop of blood with the cotton ball. Place a drop of blood about 2 cm from the end of a clean microscope slide.

7. Use a second slide to spread the blood across the first slide, as illustrated in figure 38.1. Discard the slide used for spreading the blood in the appropriate container.

8. Place the blood slide on a slide staining rack and let it dry in the air.

9. Put enough Wright's stain on the slide to cover the smear but not overflow the slide. Count the number of drops of stain that are used.

10. After 2-3 minutes, add an equal volume of distilled water to the stain and let the slide stand for

4 minutes. From time to time, gently blow on the liquid to mix the water and stain.

11. Flood the slide with distilled water until the blood smear appears light blue.

12. Tilt the slide to pour off the water and let the slide dry in the air.

Examine the blood smear with low-power magnification and locate an area where the blood cells are well distributed. Observe these cells using high-power magnification and then with an oil immersion objective if one is available.

agranulocytes lymphocyte monocyte platelet (thrombocyte)

4. In Part B of the laboratory report, prepare sketches of single blood cells to illustrate each type. Pay particular attention to relative size, nuclear shape, and color of granules in the cytoplasm (if present).

Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

Get My Free Ebook


Post a comment