Demonstration Rh Blood Typing

1. Review a textbook section on the Rh Blood Group.

2. Complete Part C of the laboratory report.

Table 40.1 Possible^ Reactions of ABO Blood-Typing Sera

Reactions

Blood Type

Anti-A Serum

Anti-B Serum

Clumping

No clumping

Type A

No clumping

Clumping

Type B

Clumping

Clumping

Type AB

No clumping

No clumping

Type O

3. To determine the Rh blood type of a blood sample, follow these steps:

a. Lance the tip of a finger. (See the demonstration procedures in Laboratory Exercise 38 for directions.) Place a small drop of blood in the center of a clean microscope slide.

b. Add a drop of anti-D serum to the blood and mix them together with a clean toothpick.

c. Place the slide on the plate of a warming box (Rh blood-typing box or Rh view box) that has been prewarmed to 45°C (113°F) (fig. 40.2).

d. Slowly rock the box back and forth to keep the mixture moving, and watch for clumping of the blood cells. When clumping occurs in anti-D serum, the clumps usually are smaller than those that appear in anti-A or anti-B sera, so they may be less obvious. However, if clumping occurs, the blood is called Rh positive; if no clumping occurs within

2 minutes, the blood is called Rh negative.

e. Discard all contaminated materials in appropriate containers.

4. Complete Part D of the laboratory report.

Figure 40.2 Slide warming box used for Rh blood typing.

Figure 40.2 Slide warming box used for Rh blood typing.

Blood Cranium
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Essentials of Human Physiology

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