Blood Testing A Demonstration

MATERIALS NEEDED

textbook sterile disposable blood lancets sterile absorbent cotton 70% alcohol

For Demonstration A:

heparinized microhematocrit capillary tube sealing clay (or Critocaps) microhematocrit centrifuge microhematocrit reader

For Demonstration B:

hemoglobinometer lens paper hemolysis applicator

For Demonstration C:

hemocytometer

Unopette system (a Becton Dickinson product) for counting red blood cells (see Instructor's Manual for a supplier) hand counter (tally)

For Demonstration D:

hemocytometer

Unopette system (a Becton Dickinson product) for counting white blood cells (see Instructor's Manual for a supplier) hand counter (tally)

SAFETY

• It is important that students learn and practice correct procedures for handling body fluids. Consider using either mammal blood other than human or contaminant-free blood that has been tested and is available from various laboratory supply houses. Some of the procedures might be accomplished as demonstrations only. If student blood is utilized, it is important that students handle only their own blood.

• Use an appropriate disinfectant to wash the laboratory tables before and after the procedures.

• Wear disposable gloves when handling blood samples.

• Clean the end of a finger with 70% alcohol before the puncture is performed.

• The sterile blood lancet should be used only once.

• Dispose of used lancets and blood-contaminated items into an appropriate container (never use the wastebasket).

• Wash your hands before leaving the laboratory.

As an aid in identifying various disease conditions, tests are often performed on blood to determine how its composition compares with normal values. These tests commonly include red blood cell percentage, hemoglobin content, red blood cell count, and total white blood cell count. Common tests performed on the blood and factors that can influence the normal values can be found in a textbook.

Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

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