Abo Blood Typing

1. Review a textbook section on the ABO blood group.

2. Compete Part A of Laboratory Report 40.

3. Perform the ABO blood type test using the blood-typing kit. To do this, follow these steps:

a. Obtain a clean microscope slide and mark across its center with a wax pencil to divide it into right and left halves. Also write "anti-A" near the edge of the left half and "anti-B" near the edge of the right half (fig. 40.1).

b. Place a small drop of blood on each half of the microscope slide. Work quickly so that the blood will not have time to clot.

How Separet The Blood From The Serum

c. Add a drop of anti-A serum to the blood on the left half and a drop of anti-B serum to the blood on the right half. Note the color coding of the anti-A and anti-B typing sera. To prevent contaminating the serum, avoid touching the blood with the serum while it is in the dropper; instead allow the serum to fall from the dropper onto the blood.

d. Use separate toothpicks to stir each sample of serum and blood together, and spread each over an area about as large as a quarter. Dispose of toothpicks in an appropriate container.

e. Examine the samples for clumping of blood cells (agglutination) after 2 minutes.

f. See table 40.1 for aid in interpreting the test results.

g. Discard contaminated materials as instructed by the laboratory instructor.

4. Complete Part B of the laboratory report.

Critical Thinking Application

I Judging from the observations of the blood-typing results, predict the components in the anti-A and anti-B sera that caused clumping.

Table 40.1 Possible^ Reactions of ABO Blood-Typing Sera

Essentials of Human Physiology

Essentials of Human Physiology

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Responses

  • kiera
    Which components in the antiA and antiB sera caused clumping?
    5 years ago
  • amaranto cremonesi
    What components in antiA and Antib sera cause agglutination?
    4 years ago
  • asmara yohannes
    Which components in the antiA and antiB sera caused agglutination?
    4 years ago

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