Replication with an Unchanging Separate Source of Small Delta Protein

Because of some of the problems of delta protein heterogeneity described above in Sect. 3.1.2, a recent study has established a cell system in which HDV replication occurs in the presence of an unchanging DNA-directed source of S-HDAg (Chang et al. 2005a). In this system an integrated cDNA provides a single source, under tetracycline control, of S-HDAg. Added to these cells is an HDV RNA genome previously modified so that it can no longer express any S-HDAg. This new system provides a better approach to address important questions of HDV replication questions. Some applications will be described in subsequent Sections.

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