The Template

In 1970, Oscar Miller, Jr., Barbara Hamkalo, and Charles Thomas used electron microscopy to examine cellular contents and demonstrate that RNA is transcribed from a DNA template. The results of this study revealed within the cell the presence of Christmas-tree-like structures: thin central fibers (the trunk of the tree), to which were attached strings (the branches) with granules (IFigure 13.3). The addition of deoxyribonuclease (an enzyme that degrades DNA) caused the central fibers to disappear, indicating that the "tree trunks" were DNA molecules. Ribonuclease (an enzyme that degrades RNA) removed the granular strings, indicating that the branches were RNA. Their conclusion was that each Christmas tree represented a gene undergoing transcription. The transcription of each gene begins at the top of the tree; there, little of the DNA has been transcribed and the RNA branches are short. As the transcription apparatus moves down the tree, transcribing more of the template, the RNA molecules lengthen, producing the long branches at the bottom.

4 13.3 Under the electron microscope, DNA molecules undergoing transcription exhibit Christmas-tree-like structures. The trunk of each "Christmas tree" (a transcription unit) represents a DNA molecule; the tree branches (granular strings attached to the DNA) are RNA molecules that have been transcribed from the DNA. As the transcription apparatus moves down the DNA, transcribing more of the template, the RNA molecules become longer and longer. (O. L. Miller, B. R. Beatty, D. W. Fawcett/Visuals Unlimited.)

The transcribed strand The template for RNA synthesis, as for DNA synthesis, is a single strand of the DNA double helix. Unlike replication, however, transcription typically takes place on only one of the two nucleotide strands of DNA (IFigure 13.4). The nucleotide strand used for transcription is termed the template strand. The other strand, called the nontemplate strand, is not ordinarily transcribed. Thus, in any one section of DNA, only one of the nucleotide strands normally carries the genetic information that is transcribed into RNA (there are some exceptions to this rule).

Evidence that only one DNA strand serves as a template came from several experiments carried out by Julius

Template strand

0 0

Post a comment