RNA splicing takes place within the spliceosome

at particular locations within it. By attaching fluorescent tags to pre-mRNA and using special imaging techniques, researchers have been able to observe the location of pre-mRNA as it is transcribed and processed. The results of these studies revealed that intron removal and other processing reactions take place at the same sites as those of transcription (IFigure 14.13), suggesting that these processes may be physically coupled. This suggestion is supported by the observation that part of RNA poly-merase II is also required for the splicing and 3' processing of pre-mRNA.

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