Physical Maps

Physical maps are based on the direct analysis of DNA, and they place genes in relation to distances measured in number of base pairs, kilobases, or megabases (I Figure 19.3). A common type of physical map is one that connects isolated pieces of genomic DNA that have been cloned in bacteria or yeast. Physical maps generally have higher resolution and are more accurate than genetic maps. A physical map is analogous to a neighborhood map that shows the location of every house along a street, whereas a genetic map is analogous to a highway map that shows the locations of major towns and cities.

A number of techniques exist for creating physical maps, including restriction mapping, which determines the positions of restriction sites on DNA; sequence-tagged site (STS) mapping, which locates the positions of short unique

|This map shows the actual location of the genes. It has greater resolution and accuracy,.

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r_glki c-chai his4

^ .whereas this map is created from recombination frequency data and has limited accuracy.

SUP53 leu2

Centromere

--peti8

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