Locating Genes That Affect Quantitative Characteristics

The statistical methods described for use in analyzing quantitative characteristics can be used both to make predictions about the average phenotype expected in offspring and to estimate the overall contribution of genes to variation in the characteristic. These methods do not, however, allow us to identify and determine the influence of individual genes that affect quantitative characteristics. The genes that control polygenic characteristics are referred to as quantitative trait loci (QTLs). Although quantitative genetics has made important contributions to basic biology and to plant and animal breeding, the inability to identify QTLs and measure their individual effects has severely limited the application of quantitative genetic methods.

Mapping QTLs In recent years, numerous genetic markers have been identified and mapped with the use of recombinant DNA techniques, making it possible to identify QTLs by linkage analysis. The underlying idea is simple: if the inheritance of a genetic marker is associated consistently with the inheritance of a particular characteristic (such as increased height), then that marker must be linked to a QTL that affects height. The key is to have enough genetic markers so that QTLs can be detected throughout the genome. With the introduction of restriction fragment length polymorphisms and microsatellite variations (see pp. 000 and pp. 000 in Chapter 18), variable markers are now available for mapping QTLs in a number of different organisms (I Figure 22.19).

A common procedure for mapping QTLs is to cross two homozygous strains that differ in alleles at many loci. The resulting Fj progeny are then intercrossed or backcrossed to allow the genes to recombine through independent assortment and crossing over. Genes on different chromosomes and genes that are far apart on the same chromosome will recombine freely; genes that are closely linked will be inherited together. The offspring are measured for one or more quantitative characteristics; at the same time, they are geno-typed for numerous genetic markers that span the genome. Any correlation between the inheritance of a particular marker allele and a quantitative phenotype indicates that a QTL is linked to that marker. If enough markers are used, it is theoretically possible to detect all the QTLs affecting a characteristic. This approach has been used to detect genes affecting various characteristics in several plant and animal species (Table 22.2).

Applications of QTL mapping The number of genes affecting a quantitative characteristic can be estimated by

122.19 The availability of molecular markers makes the mapping of QTLs possible in many organisms.

1 Table 22.21

Quantitative characteristics for

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