Anu

Homologous recombination

.where the disabled copy recombines with the normal gene on the mouse chromosome.

neo+

Mouse neo+ cells

^ The cells are grown on a medium that contains the antibiotic G418 and gancyclovir; only cells that received the neo+ gene by homologous recombination survive.

neo+

Mouse neo+ cells

White mouse embryo

^ The cells are grown on a medium that contains the antibiotic G418 and gancyclovir; only cells that received the neo+ gene by homologous recombination survive.

yfc Variegated progeny contain a mixture of normal cells and cells with the disabled gene.

.which are implanted into a pseudopregnant mouse.

Variegated mice are then interbred and produce some progeny that are homozygous for the knocked-out gene.

Conclusion: Through this procedure, mice that contain no functional copy of the gene—that is, the gene is knocked out—are produced. The phenotype of the knockout mice reveals the function of the gene.

White mouse embryo

.which are implanted into a pseudopregnant mouse.

yfc Variegated progeny contain a mixture of normal cells and cells with the disabled gene.

Variegated mice are then interbred and produce some progeny that are homozygous for the knocked-out gene.

Conclusion: Through this procedure, mice that contain no functional copy of the gene—that is, the gene is knocked out—are produced. The phenotype of the knockout mice reveals the function of the gene.

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