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Exon 2

| The 3'-OH group of a guanine nucleotide attacks and cleaves the 5' end of the intron.

5 ' splice site

[2 The guanine nucleotide is added to the 5' end of the intron,.

^ .and a free 3'-OH group is generated at the end of exon 1.

3'-OH at the end of exon 1 attacks the 5' end of exon 2,.

^ .cleaving the intron at its 3' end, releasing the intron, and splicing the two exons together.

Intron

3' splice site

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