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Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.

DNA from a strain of Bacillus subtilis with the genotype trp+ tyr+ is used to transform a recipient strain with the genotype trpr tyrr. The following numbers of transformed cells were recovered:

Genotype Number of transformed cells trp+ tyrr 154

trpr tyr+ 312

What do these results suggest about the linkage of the trp and tyr genes?

DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype ar br cr dr er. Pairs of genes are checked for cotransformation and the following results are obtained:

Pair of genes and b+ and c+ and d+ and e+ and c+ and d+ and e+ and d+ and e+ and e+

Cotransformation no no yes yes yes no yes no yes no

On the basis of these results, what is the order of the genes on the bacterial chromosome?

21. DNA from a bacterial strain that is his+ leu+ lac+ is used to transform a strain that is his- leu~ lac-. The following percentages of cells were transformed:

Donor Strain

Recipient strain

Genotype of transformed his+ leu+ lac+ his leu lac

Genotype of transformed his+ leu+ lac+ his leu lac

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