^ Newly synthesized octamers are less dense and thus will be higher in the tube.

Single band; old octamers with heavy amino acids

Isolate octamers

Old octamers are dense and will move toward the bottom of the tube. J Broad band;

octamers with mixture of old and new histones (heavy and light amino acids)

Conclusion: After DNA replication, the new octamers are a random mixture of old and new histones.

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