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A continuous sequence of nucleotides in the DNA.

A continuous sequence of nucleotides in the DNA.

mRNA 5'

PĀ£ePtideBA*"9 [Gy Tyr HPheVal fei]

AminOacids

14.1 The concept of colinearity suggests that a continuous sequence of nucleotides in DNA encodes a continuous sequence of amino acids in a protein.

The concept of colinearity suggests that the number of nucleotides in a gene should be proportional to the number of amino acids in the protein encoded by that gene. In a general sense, this concept is true for genes found in bacterial cells and many viruses, although these genes are slightly longer than expected if colinearity is strictly applied (the mRNAs encoded by the genes contain sequences at their ends that do not specify amino acids). At first, eu-karyotic genes and proteins also were generally assumed to be colinear, but there were hints that eukaryotic gene structure was fundamentally different. Eukaryotic cells contain far more DNA than is required to encode proteins (the so-called C-value paradox; see Chapter 11). Furthermore, many large RNA molecules observed in the nucleus were absent from the cytoplasm, suggesting that nuclear RNAs undergo some type of change before they are exported to the cytoplasm.

Most geneticists were nevertheless surprised by the announcement in the 1970s that four coding sequences in a gene from a eukaryotic virus were interrupted by nucleotides that did not specify amino acids. This discovery was made when the viral DNA was hybridized with the mRNA transcribed from it, and the hybridized structure was examined with the use of an electron microscope (I Figure 14.2). The DNA was clearly much longer than the mRNA, because regions of DNA looped out from the hybridized molecules. These regions contained nucleotides in the DNA that were absent from the coding nucleotides in the mRNA. Many other examples of interrupted genes were subsequently discovered; it quickly became apparent that most eukaryotic genes comprise stretches of coding and noncoding nucleotides.

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