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2\ Different DNA molecules are cut in different places, providing a set of overlapping fragments

2\ Different DNA molecules are cut in different places, providing a set of overlapping fragments a

3| Each fragment is then joined to a cloning vector.

. and transferred to a bacterial cell,.

3| Each fragment is then joined to a cloning vector.

. and transferred to a bacterial cell,.

| .producing a set of clones containing overlapping genomic fragments, some of which may include segments of the gene of interest.

| .producing a set of clones containing overlapping genomic fragments, some of which may include segments of the gene of interest.

Conclusion: Some clones contain the entire gene of interest, others include part of the gene, and most contain none of the gene of interest.

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