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F'

Present as separate circular DNA, carrying some bacterial genes

Donor

Conjugating Cells

Cell Types Present After Conjugation

Two F+ cells (F~ cell becomes F+) One F+ cell and one F~ (no change)* Two F' cells (F~ cell becomes F')

*Rarely, the F cell becomes F+ in an Hfr x F conjugation if the entire chromosome is transferred during conjugation.

one on the bacterial chromosome and one on the newly introduced F plasmid. The outcomes of conjugation between different mating types of E. coli are summarized in Table 8.3.

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