(a) Determine the order of the three genes on the phage chromosome.

(b) Determine the map distances between the genes.

(c) Determine the coefficient of coincidence and the interference (see p. 000 in Chapter 7).

A donor strain of bacteria with genes a+ b+ c+ is infected with phages to map the donor chromosome with generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a- b- c-. Bacteria with the a+ gene are selected and the percentage of cells with cotransduced b+ and c+ genes are recorded.

Selected Cells with

Donor Recipient gene cotransduced gene (%)

Is the b or c gene closer to a? Explain your reasoning.

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