Bam HI site EcoRI site

2 kb

7 kb

1 kb

3 kb

In the example in the Worked Problem, we can map the restriction sites in our heads or with a few simple sketches. Most restriction mapping is done with several restriction enzymes, used alone and in various combinations, producing many restriction fragments. With long pieces of DNA (greater than 30 kb), computer programs are used to determine the restriction maps, and restriction mapping may be facilitated by tagging one end of a large DNA fragment with radioactivity or by identifying the end with the use of a probe.

Physical maps, such as restriction maps of DNA fragments or even whole chromosomes, are often created for genomic analysis. These lengthy maps are often put together by combining maps of shorter, overlapping genomic fragments.

Was this article helpful?

0 0

Post a comment