.and no plaques are formed.

Conclusion: The complementation test indicates whether two mutations occur at the same locus or at different loci.

8.32 Complementation tests are used to determine whether different mutations are at the same functional gene.

a+ br were effectively heterozygous for the phage genes, with the mutations in the trans configuration ( FIGURE 8.32, step 2). In the complementation testing, the phenotypes of progeny phages were examined on the K strain, rather than the B strain as illustrated in Figure 8.31.

Gene B lies entirely within gene A.

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