Lan

Intron

5 ' splice site

Intron

[2 The guanine nucleotide is added to the 5' end of the intron,.

^ .and a free 3'-OH group is generated at the end of exon 1.

3'-OH at the end of exon 1 attacks the 5' end of exon 2,.

GPU Exon2 3'

Croup I intron

Conclusion: A group I intron is removed through a unique self-splicing reaction.

14.14 Group I introns undergo self-splicing. (a) Secondary structure of a group I intron. (b) Self-splicing of a group I intron.

Exon I 5' 3' Exon II Group II intron

Exon I 5' 3' Exon II Group II intron

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