Ii I I

Contig

Gene A

| .which allows the large-insert clones to be assembled into a contig, a continuous stretch of DNA.

A subset of overlapping clones that cover the entire chromosome are selected and fractured. These pieces are then cloned.

| .which allows the large-insert clones to be assembled into a contig, a continuous stretch of DNA.

A subset of overlapping clones that cover the entire chromosome are selected and fractured. These pieces are then cloned.

y Subclones

^ The final sequence is assembled by putting together the sequences of the large clones and filling in any gaps.

Gene A

Gene B

Gene C

Gene D

rt Fach of these small-insert clones is sequenced, and overlap in sequences is used to assemble them in the correct order.

Gene B

Gene C

Gene D

y Subclones

^ The final sequence is assembled by putting together the sequences of the large clones and filling in any gaps.

4 19.11 Map-based approaches to whole-genome sequencing rely on detailed genetic and physical maps to align sequenced fragments.

Genomic DNA

| Genomic DNA is cut into numerous small overlapping fragments and cloned in bacteria.

Each fragment is sequenced.

| Overlap in sequence is used to order the clones,.

Genomic DNA

| Genomic DNA is cut into numerous small overlapping fragments and cloned in bacteria.

| Overlap in sequence is used to order the clones,.

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