Genomic and cDNA libraries may be screened with a probe to find the gene of interest

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When part of the DNA sequence of the gene has been determined, a set of DNA probes can be synthesized chemically by using an automated machine known as an oligonucleotide synthesizer. The resulting probes can be used to screen a library for a gene of interest.

Yet another method of screening a library is to look for the protein product of a gene. This method requires that the DNA library be cloned in an expression vector. The clones can be tested for the presence of the protein by using an antibody that recognizes the protein or by using a chemical test for the protein product. This method depends on the existence of a test for the protein produced by the gene.

Almost any method used to screen a library will identify several clones, some of which will be false positives that do not contain the gene of interest; several screening methods may be needed to determine which clones actually contain the gene. __

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