The Ames test uses his- strains of bacteria to test chemicals for their ability to produce his-: his+ mutations. Because mutagenic activity and carcinogenic potential are closely correlated, the Ames test is widely used to screen chemicals for their cancer-causing potential.

„a his bacteria

| Bacterial his- strains are mixed with liver enzymes (which have the ability to convert compounds into potential mutagens).

Some of the bacterial strains are also mixed with the chemical to be tested for mutagenic activity.

The bacteria are then plated on medium that lacks histidine.

The bacteria are then plated on medium that lacks histidine.


Incubate m

Control plate (no chemical)

| Bacterial colonies that appear on the plates have undergone a his~-*- his + mutation.

Treatment plate (chemical added)

of colonies appearing on the plate is mutagenic and therefore probably also carcinogenic.

Was this article helpful?

0 0
10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook

Post a comment