Concepts

Particular mutations can be introduced at specific sites within a gene by means of site-directed mutagenesis.

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Transgenic animals The oocytes of mice and other mammals are large enough that DNA can be injected into them directly. Immediately after penetration by sperm, il A short sequence of nucleotides is cut out. >

a and replaced by a synthetic sequence containing mutated bases.

il A short sequence of nucleotides is cut out. >

Recombinant plasmid DNA

DNA ligase

Recombinant plasmid DNA

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