Concepts

After DNA replication, new nucleosomes quickly reassemble on the molecules of DNA. Nucleosomes apparently break down in the course of replication and reassemble from a random mixture of old and new histones.

Location of replication within the nucleus The DNA

polymerases that carry out replication are frequently depicted as moving down the DNA template, much as a locomotive travels along a train track. Recent evidence suggests that this view is incorrect. A more accurate view is that the polymerase is fixed in location, and template DNA is threaded through it, with newly synthesized DNA molecules emerging from the other end.

Question: What happens to histones during eukaryotic DNA replication?

ffi Grow cells for several generations in medium that contains amino acids labeled with a heavy isotope

2j Transfer the cells to a medium that contains amino acids labeled with a light isotope.

ffi Grow cells for several generations in medium that contains amino acids labeled with a heavy isotope

2j Transfer the cells to a medium that contains amino acids labeled with a light isotope.

Isolate histone octamers before and after replication..

Isolate octamers

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