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[3 .produces non-crossover recombinants consisting of two heteroduplex molecules.

fT4 .produces crossover '

recombinants consisting of two heteroduplex molecules.

Conclusion: The Holliday model predicts non-crossover or crossover recombinant DNA, depending on whether cleavage is in the horizontal or the vertical plane.

Cross bridges rt Two double-stranded DNA molecules from homologous chromosomes align.

A double-strand break occurs in one of the molecules.

A double-strand break occurs in one of the molecules.

Cross bridges

^ Strand attachment produces two Holliday junctions, which can each be separated by cleavage and reunion.

^ Strand attachment produces two Holliday junctions, which can each be separated by cleavage and reunion.

12.24 In the double-strand-break model, recombination is accomplished through a double-strand break in one DNA duplex, strand displacement, DNA synthesis, and resolution of two Holliday junctions.

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