Mej

ddATP

ddTTP

ddCTP

ddGTP

Origin

In the dideoxy sequencing reaction, the original fragment is used as a template for the synthesis of a new DNA strand; and it is the sequence of the new strand that is actually determined. The first task, therefore, is to write out the sequence of the newly synthesized fragment, which will be complementary and antiparallel to the original fragment. The sequence of the newly synthesized strand, written 5' : 3' is: 5'-GATGCTAAGC-3'. Bands representing this sequence will appear on the gel, with the bands representing nucleotides near the 5' end of the molecule at the bottom of the gel.

The New Genetics

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