Kursad Turksen

Ottawa Health Research Institute Ottawa, Ontario, Canada

Humana Press ^ Totowa, New Jersey

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Cover illustration: Figure 1 from Chapter 16, "Genetic Manipulation of Human Embryonic Stell Cells by Transfection," by Rachel Eiges. Figure 2 from Chapter 2, "Blastocyst Culture for Deriving Human Embryonic Stem Cells," by Ariff Bongso.

Production Editor: Jennifer Hackworth Cover design by Patricia F. Cleary

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Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1

eISBN: 1-59745-046-4 ISSN: 1064-3745

Library of Congress Cataloging-in-Publication Data

Human embryonic stem cell protocols / edited by Kursad Turksen.

p. ; cm. -- (Methods in molecular biology, ISSN 1064-3745 ; v. 331) Includes bibliographical references and index. ISBN 1-58829-497-8 (alk. paper)

1. Embryonic stem cells--Laboratory manuals. 2. Human embryo--Laboratory manuals.

[DNLM: 1. Stem Cells. 2. Embryo Research. QU 325 H918 2006] I. Turksen, Kursad. II. Series: Methods in molecular biology (Clifton, N.J.) ; v. 331. QH588.S83H856 2006 616'.02774--dc22


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