Histological Analysis

2.5.1. Processing Tissue for Histological Analysis

1. Dissecting tools: fine-tipped Dumont forceps (Fine Science tools, Vancouver, Canada; cat. no. 11251-30), straight fine scissors 10.5 cm (Fine Science tools; cat. no. 14094-11).

2. 50-mL sterile tubes (Greiner, Bio-One; cat. no. 227270).

3. Neutral buffered formalin (NBF): 40% formaldehyde (Bio-Lab, Jerusalem, Israel; cat. no. 72026), 46 mM Na2HPO4 (Merck, Whitehouse Station, NJ; cat. no. 10686),

33 mM NaH2PO4 (CARLO ERBA Reagenti, Milano, Italy; cat. no. 480087). For 3 L of NBF: combine 300 mL of 40% formalin, 12 g Na2HPO4-2H2O, 19.5 g Ma2HPO4 anhydrous and 2700 mL of H2O.

4. 70, 95, and 100% ethanol (see Subheading 2.3., item 6); solutions prepared with double distilled H2O.

6. Embedding wax: Paraplast Plus Wax (Kendall Tyco Health Care Group, Mansfilled MA; cat. no. 502004).

7. Microtome (Leica, Bensheim, Germany; model no. LeicaRM 2135).

8. Poly-l-lysine-coated slides, Super FrostPlus (Manzel-Glaser, Braunschweig, Germany; cat. no. MG051101).

2.5.2. Hematoxylin and Eosin Staining of Paraffin Sections

1. Coplin Jar (Finkelman, Petach Tikva, Israel; cat. no. FNK 25146).

4. Hematoxylin solution (Merck; cat. no. 4305).

5. 1% Eosin-Y (Harleco, Kansas City, MO; cat. no. Na 45380) in 95% ethanol.

6. Upright Microscope CX31 equipped with DP70 camera (Olympus, Lake Success, NY).

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