Acknowledgment

We gratefully acknowledge BresaGen, Inc. for giving us the opportunity to isolate four BresaGen human ES cell lines. We also would like to thank our collaborators at Wisconsin Hygiene State Cytogenetic Laboratory, Dr. Lorraine Meisner and Julie Johnson, for help in developing karyotype protocols as well as fingerprinting the hES cell lines. Our special thanks to Human IVF laboratory at RBA in Atlanta, especially Dr. David Wininger, Emy Jones, and Thomas Elliott, for contributing their expertise in the culture and evaluation of early embryos.

References

1 Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147.

2 Thomson, J. A., Kalishman, J., Golos, T. G., et al. (1995) Isolation of a primate embryonic stem cell line. Proc. Natl. Acad. Sci. USA 92, 7844-7848.

3 Thomson, J. A., Kalishman, J., Golos, T. G., Durning, M., Harris, C. P., and Hearn, J. P. (1996) Pluripotent cell lines derived from common marmoset (Callithrix jac-chus) blastocysts. Biol. Reprod. 55, 254-259.

4 Richards, M., Fong, C. Y., Chan, W. K., Wong, P. C., and Bongso, A. (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat. Biotechnol. 20, 933-936.

5 Mitalipova, M., Calhoun, J., Shin, S., et al. (2003) Human embryonic stem cell lines derived from discarded embryos. Stem Cells 21, 521-526.

6 Draper, J. S., Smith, K., Gokhale, P., et al. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol. 22, 53-54.

7 Buzzard, J. J., Gough, N. M., Crook, J. M., and Colman, A. (2004) Karyotype of human ES cells during extended culture. Nat. Biotechnol. 22, 381-382.

8 Mitalipova, M. M., Rao, R. R., Hoyer, D. M., et al. (2005) Preserving the genetic integrity of human embryonic stem cells. Nat. Biotechnol. 23, 19-20.

9 Solter, D. and Knowles, B. B. (1975) Immunosurgery of mouse blastocyst. Proc. Natl. Acad. Sci. USA 72, 5099-5102.

10 Amit, M. and Itskovitz-Eldor, J. (2004) Isolation and maintenance of primate ES cells. Handbook of Stem Cells, Vol. 1, pp. 419-436.

11 Klimanskaya, I. and McMahon, J. (2004) Approaches for derivation and maintenance of human ES cells: detailed procedures and alternatives. Handbook of Stem Cells, Vol. 1, pp. 437-449.

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