The three macrodroplet formats are shown to work effectively with actinomy-cetes. Application of this approach to other ''biosynthetically talented'' organisms such as fungi and myxobacteria is explored. Both are useful sources of chemical diversity and much is known about their genetics. We have done some preliminary combinatorial biology on both types of organisms. Since both types are used to produce natural products through fermentation, the macrodroplet approach is adopted to screen the combinatorial clones.
To supplement the E. coli-based assay targets, encapsulation of actinomy-cetes has been used in the plate assay format to produce zones of clearing on lawns of fungal spores. We have used lawns of other prokaryotes in the plate format including Staphylococcus aureus, various engineered E. coli strains, En-terococcus faecalis, Sarcina aurantica, and Bacillus subtilis. In addition, engineered mammalian cell lines were also used in the plate assay format in this way. The macrodroplets can be used to screen clones or chemical libraries against a range of lawns from enzymes to insects and almost anything in between.
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