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Classical cAMP assays involved isolation of cAMP in acid extracts from cells (by first preparing cell extracts) and quantitation by ELISA assays. Now, several homogeneous and heterogeneous cAMP kits are available based on different technologies from different vendors. Some of those are given here.

Classical cAMP assays involved isolation of cAMP in acid extracts from cells (by first preparing cell extracts) and quantitation by ELISA assays. Now, several homogeneous and heterogeneous cAMP kits are available based on different technologies from different vendors. Some of those are given here.

Figure 14 SPA cAMP assay for screening signal transduction via adenyl cyclase. (A) Cyclic AMP standard curve. (B) Activation of adenyl cyclase by agonist of GPCR. CHO-K1 cells expressing GPCR, when treated with agonist activates Gas and adenyl cyclase and consequently increases intracellular cAMP. There is a good dose-response of agonist in stimulating cAMP levels.

Figure 14 SPA cAMP assay for screening signal transduction via adenyl cyclase. (A) Cyclic AMP standard curve. (B) Activation of adenyl cyclase by agonist of GPCR. CHO-K1 cells expressing GPCR, when treated with agonist activates Gas and adenyl cyclase and consequently increases intracellular cAMP. There is a good dose-response of agonist in stimulating cAMP levels.

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