plexes formed upon binding of biotin and streptavidin decreases (complex %). This value provides the key additional information that allows the high value in this well to be discarded as a false positive. Namely, the high value can be attributed to the failure of a dispenser to deliver the appropriate amount of streptavidin to this well. The resulting lower volume in this well results in the increase in count rate and particle number (the final concentration of biotin is high due to the lower volume), while the lower amount of streptavidin results in a decrease in the amount of complex formed. This example shows how the simultaneous analysis of multiple parameters helps improve the efficiency and precision of screening runs by helping to eliminate false positive results rapidly and effectively.
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