Marcel

Table 4 Cyclic AMP Assays

Assay Rad/nonrad Assay type Reagents Readout Provider

Table 4 Cyclic AMP Assays

Assay Rad/nonrad Assay type Reagents Readout Provider

ELISA assays

Nonradioactive

Heterogeneous

cAMP-ab, 2°-ab-coated plate, cAMP conjugated with HRP or alk. phos.

Colorimetric, fluorescence plate reader

Sigma and several other vendors

Radioimmuno assay

Radioactive

Heterogeneous

[3H] or [125I]-cAMP, cAMP-ab coated bead

Radioactive counting y-counter or ß-plate counter

Amersham, NEN

FLISA assay

Nonradioactive

Homogeneous

biotin cAMP, cAMP-ab coated bead, Cy5-streptavidin

CY5 Fluorescence based FMAT reader

PE Biosystems

Fluorescence Polariza

Nonradioactive

Homogeneous

Fluorescent cAMP,

FP-plate reader

Was this article helpful?

0 0

Post a comment