Marcel

Figure 1 Inhibition curve for tranylcypromine inhibition of CYP2C19. The assay was conducted as described in Table 1. The experimental design can be varied as desired. The 12 wells in the row of a 96-well plate can be used for one inhibition curve. Wells 1 to 8 contain serial 1: 3 dilutions of the inhibitors (50 ||L transferred between wells containing 100 ||L). Wells 9 and 10 contain no inhibitor, and rows 11 and 12 are blanks for background fluorescence (a stop solution is added before the enzyme). Data was acquired using a Cytofluor model 2350 fluorescent plate scanner.

Figure 1 Inhibition curve for tranylcypromine inhibition of CYP2C19. The assay was conducted as described in Table 1. The experimental design can be varied as desired. The 12 wells in the row of a 96-well plate can be used for one inhibition curve. Wells 1 to 8 contain serial 1: 3 dilutions of the inhibitors (50 ||L transferred between wells containing 100 ||L). Wells 9 and 10 contain no inhibitor, and rows 11 and 12 are blanks for background fluorescence (a stop solution is added before the enzyme). Data was acquired using a Cytofluor model 2350 fluorescent plate scanner.

Was this article helpful?

0 0

Post a comment