in Fig. 8b. (3) NanoStore, a rapid, miniaturized multireplica compound repository providing the link between traditional single-compound library formats and EVOscreen™. (4) A microseparation device (HPLC) coupled to the detection system. (5) A scanner and picker device for the analysis of combinatorial libraries and for functional genome analysis (described below).

1. Liquid Handling

The ability to miniaturize assays to the submicroliter level is largely dependent on the precision of liquid handling systems. Modern pipetting and dispensing systems enable components to be reproducibly added in volumes in the nanoliter range of thousands of samples per plate (Fig. 9). The EVOscreen™ system in-

Figure 9 A plot demonstrating the linearity of performance of the liquid dispensing system in the low nanoliter range.

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