M A R C B L

Figure 3 Nonideal progress curves from typical enzyme assays: normal linear (a), lag (b), initial burst (c), unstable enzyme (d), and autocatalytic (e).

therefore interfering compounds can often be weeded out because they would affect only the intercept and not the slope. This requires that the compound shifts the initial and final signal by the same absolute amount, which is not necessarily the case with fluorescence detection. It is also of value to examine the initial time point to see if there is a dramatic effect of the compound on the assay signal when there is minimal product formed.

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