Product (R) detected by absorbence or fluorescence

Capture biotinylated components on streptavldin coated SPA beads or Flash Plates™

3. Biotiir—v-°L^,Fluores. Prolease> Biotitrvx^- + Fluores.

Add streptavldin and measure fluorescence polarization

Measure appearance of EDANS


Add streptavidln-APC and measure resonance energy transfer from Cy-5 to A PC.

Figure 2 Common assay schemes for protease targets. These schemes are amenable to ''mix and measure'' formats that are preferable in HTS.

Figure 3 Common assay schemes for kinase and phosphatase targets compatible ''mix and measure'' formats.

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