Product (R) detected by absorbence or fluorescence
Capture biotinylated components on streptavldin coated SPA beads or Flash Plates™
3. Biotiir—v-°L^,Fluores. Prolease> Biotitrvx^- + Fluores.
Add streptavldin and measure fluorescence polarization
Measure appearance of EDANS
Add streptavidln-APC and measure resonance energy transfer from Cy-5 to A PC.
Figure 2 Common assay schemes for protease targets. These schemes are amenable to ''mix and measure'' formats that are preferable in HTS.
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