A

Bio- Peptide-OH

Bio- Peptide-OH

anti-PY-MAb, nM

Figure 17 Origen PTK assay. (A) Schematic representation of PTK assay. A biotin-peptide is phosphorylated by PTK. The phosphorylated biotin-peptide is bound to strepta-vidin coated magnetic beads and will give signal by association with TAG labeled PY antibody. (B) Titration with anti-PY antibody in the Origen PTK assay. The S/N of 200 is observed at about 1.8 nM of PY antibody. (Courtesy of IGEN, Inc.)

anti-PY-MAb, nM

Figure 17 Origen PTK assay. (A) Schematic representation of PTK assay. A biotin-peptide is phosphorylated by PTK. The phosphorylated biotin-peptide is bound to strepta-vidin coated magnetic beads and will give signal by association with TAG labeled PY antibody. (B) Titration with anti-PY antibody in the Origen PTK assay. The S/N of 200 is observed at about 1.8 nM of PY antibody. (Courtesy of IGEN, Inc.)

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