Optimum extraction conditions differ for different types of metabolites. To extract carbohydrates, amino acids, and fatty acids from tomato tissues using GC-MS, an extraction protocol originally developed for potato tuber (Roessner et al. 2000) has been widely used. To extract secondary metabolites for non-targeted analysis by liquid chromatography MS (LC-MS), a methanol extraction has been widely applied (Bino et al. 2005; Moco et al. 2006). A lipophilic solvent (e.g., chloroform) is required for extracting non-polar secondary metabolites, such as carotenoids. To extract volatile metabolites, solid phase micro-extraction (Tikunov et al. 2005) or solvent extraction (e.g., propanol, pentane) (Schmelz et al. 2003) have been reported.
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WHAT IT IS A three-phase plan that has been likened to the low-carbohydrate Atkins program because during the first two weeks, South Beach eliminates most carbs, including bread, pasta, potatoes, fruit and most dairy products. In PHASE 2, healthy carbs, including most fruits, whole grains and dairy products are gradually reintroduced, but processed carbs such as bagels, cookies, cornflakes, regular pasta and rice cakes remain on the list of foods to avoid or eat rarely.