Ls and Exotic Libraries for the Analysis of Quantitative Traits

These favorable wild QTL alleles are a valuable resource for breeding programs after they have been fixed in NILs or ILs and after the superior performance of the line has been validated in comparison to the cultivated recurrent parent in replicated field experiments. From the tomato AB-QTL populations, MAS was applied to develop NILs that contain specific QTL al-leles derived from the wild donors S. habrochaites, S. pimpinellifolium, and S. peruvianum, and that are able to significantly...

With a priori Knowledge of Map Position

Once a PCR-based molecular linkage map is constructed, the need arises to develop markers in regions lacking sufficient coverage. Once these regions are identified, information from high-density molecular maps can be leveraged to develop markers that are targeted to specific regions of interest. The SGN database offers information about various tomato linkage maps covering the entire genome with more than 2,400 markers including RFLP, SSR and COS (Table 5). The sequences of RFLP markers can be...

Macronutrients Carbohydrates Insoluble Solids and Lipids

Plants contribute to the availability of dietary macronutrients including protein, fats, and carbohydrates. A large amount of data exists for these macronutrients in agronomic crops wherein these well-characterized constituents are significant crop yield components. In tomato, these constituents have been studied principally for their effects on fruit quality. Little attention has been given to their contribution to fruit nutritive value. Modification of plant carbohydrate composition is a...

QTL Finemapping and Cloning Using Introgression Lines

Each IL generally contains only a small percentage of the donor genome (less than 5 ), however these in-trogressions often influence several traits, including some undesirable ones. Therefore, it is often necessary to fine map QTL within an IL to reduce the length of the donor introgression. This allows not only the assessment of whether the effect on the phenotype is due to a single QTL or to several linked QTLs affecting the same trait, but also the verification of whether possible...

Tomato Sequence Resources

Because TILLING is a PCR-based technique, only genes for which the DNA sequence is known can be screened for mutations. In 2003, an International Tomato Genome Sequencing Project was launched to sequence the euchromatic regions of the tomato genome with the goal of fully annotating the estimated 35,000 tomato genes (Mueller etal. 2005b) (see Sect. 1.18). According to SGN, sequencing of the tomato genome is 17 complete as of February 2007. Designing PCR primers for TILLING any genomic region in...

Genetics and Drought Resistance Characteristics of Tomato Species

S. lycopersicum is mesophytic members of the species are not significantly drought or salt resistant. So while there is variability for drought and or osmotic stress resistance within S. lycopersicum, this variability is limited Foolad and Lin 1999 Srinivasa Rao et al. 2000 Fellner and Sawhney 2001 the best sources of resistance for cultivated tomato are from other species in the genus. S. pennellii and S. chilense are indigenous to arid and semi-arid environments in South America Rick 1973...

Viral Resistance

Finding useful markers for MAS of disease resistance requires several conditions. These include selection of a durable resistance source, use of characterized pathogen strains, a robust phenotyping method, sufficient polymorphism among parents, and simple genetic control of the resistance trait. Many of the resistance genes or QTLs are pathogen strain or race-specific. Therefore, it is essential to characterize the target pathogen population in order to select proper resistance sources and...

Genetic Linkage Maps

The first Mendelian tomato linkage group was published by Hedrick and Booth 1907 , where the dwarf and ovate loci were shown to be linked in an F2 cross between S. lycopersicum varieties Livingston Stone and Dwarf Aristocrat Tanksley 1993a . By 1934,20 morphological markers had been mapped to ten linkage groups. This study scored over 48,000 plants derived from crosses among ten varieties that were homozygous for spontaneous mutations MacArthur 1926,1934 . During the same decade researchers...

Maps Online

A number of the aforementioned maps are available on the internet at SGN Mueller etal. 2005a , National Center for Biotechnology Information NCBI Wheeler et al. 2004 , and http www.tomatomap.net Van Deynze et al. 2006 Tables 5-7 . The process of preparing genetic maps for display in the NCBI MapViewer includes populating the MapViewer database with ancillary information associated with the locus. This information includes the probe name, any GenBank accession s known, the UniSTS accession s for...

Bacterial Wilt

Ralstonia solanacarum is a species complex that causes bacterial wilt in tomato. The pathogen has been classified into five races, six biovars, and four phylotypes Denny 2006 . Strains of R. solanacearum are highly variable in their aggressiveness and cause variable severity of disease on tomatoes Jaunet and Wang 1999 . Therefore, it is important to use a well-characterized strain for resistance mapping studies. Sources of resistance to bacterial wilt in tomato have been identified primarily in...

Contributors

Harrison Ste 150 Seattle, WA 98119, USA anna.amen arcadiabio. com Kazusa DNA Research Institute Kazusa-Kamatari 2-6-7 Kisarazu, 292-0818, Japan kaoki kazusa.or.jp Danuta Babula Institute of Plant Genetics Polish Academy of Sciences Strzeszynska 34,60-479 Poznan Poland Angela M. Baldo USDA-ARS Plant Genetic Resources Unit and Grape Genetics Research Unit Geneva, NY 14456, USA angela.baldo ars.usda.gov Sang Woo Bang Laboratory of Plant Breeding Faculty of...